In collaboration with Turning the Tide of Antimicrobial Resistance  (TTA), IBA ​invites IBA PhD student members to the TTA Bioinformatics course Level 1.

Wednesday, January 20th 2021 – Oslo, UiO, Domus Medica, L-257

This course will give you basic knowledge on whole genome assembly, including quality assessment of raw data (FastQC), adapter / quality trimming and contig assembly. You will also learn about the differences between De novo assembly and mapping to an annotated reference genome.

Course schedule

13:00 – 13:30 Part I: Introduction to Whole Genome Sequencing

Theoretical introduction and applications in microbial whole genome sequence analysis:

    – Basic whole genome sequence assembly and bioinformatics

    – NGS read-to-reference alignment (contig assembly)

Analysis techniques covered will employ raw data from Illumina platforms (HiSeq/MiSeq).

13:30 – 13:45 Coffee break

13:45 – 15:30 Part II: Hands-on exercises

    – Quick explanation of associated file types

    – Performing quality checks with FastQC (before and after trimming)

    – Adapter and quality trimming

    – Contig assembly and mapping to reference genome

Course facilitator: Timo Lutter